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empty pegfp c1 vector  (TaKaRa)


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    Structured Review

    TaKaRa empty pegfp c1 vector
    Empty Pegfp C1 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+pegfp+c1+vector/pm34502296-316-0-3
    Average 86 stars, based on 1 article reviews
    empty pegfp c1 vector - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Drebrin Regulates Acetylcholine Receptor Clustering and Organization of Microtubules at the Postsynaptic Machinery.
    Article Snippet: After checking reflexes and shaving the hindlimb area to be injected, 25 μg DNA (1 μg/μL in PBS) of pEGFP-C1-DbnE-WT or pEGFP-C1-DbnE-142A plasmid was injected into the tibialis anterior muscle using a Hamilton syringe. .. Empty pEGFP-C1 vector (Clontech) was used as a negative control. .. The muscle was electroporated with 10 pulses of electrical current (180 V/cm) of 20 ms each at 1 s intervals using an ECM-830 electroporator (45-0052INT, BTX Harvard Apparatus, USA).

    Article Title: Golgi-derived CLASP-dependent Microtubules Control Golgi Organization and Polarized Trafficking in Motile Cells
    Article Snippet: Nontargeting siRNA (Dharmacon) was used for controls. .. Empty pEGFP-C1 vector (Clontech) served as a control for circularity quantifications. mCherry plasmid was provided by Dr. R. Tsien (San Diego, CA). .. YFP-GT (YFPGalactosyltransferase; Clontech), mCherry-GT (modified from Clontech), GFP-GM130 (gift from C. Sütterling, Irving, CA), and Cherry-Rab6 (gift from A. Akhmanova, Rotterdam, The Netherlands) were used for Golgi visualization.

    Article Title: Coronin 1C negatively regulates cell-matrix adhesion and motility of intestinal epithelial cells
    Article Snippet: Full length Coronin 1C construct in pEGFP-C1 vector were generated as described previously [ 15 ] and transfected into cells using Lipofectamine 2000 (Invitrogen) according to manufacturers protocol. .. Empty pEGFP-C1 vector (Clontech) was used as a control. .. Human Coronin 1C siGENOME duplex 4 and PTK2 siGENOME SmartPool (Dharmacon, Lafayette, CO) were used to downregulate Coronin 1C and FAK respectively.

    Article Title: Loss of Nucleolar Histone Chaperone NPM1 Triggers Rearrangement of Heterochromatin and Synergizes with a Deficiency in DNA Methyltransferase DNMT3A to Drive Ribosomal DNA Transcription
    Article Snippet: .. Empty pEGFP-C1 vector was purchased from Clontech. .. Immunoblotting For analysis of detergent-soluble proteins we used the Nonidet P-40 lysis buffer composed of 50 m m Tris-HCl, pH 7.5, 150 m m NaCl, 0.5% Nonidet P-40, 50 m m NaF, 1 m m NaVO 3 , 1 m m dithiothreitol, 1× protease inhibitor mixture (Roche), and 1 m m phenylmethylsulfonyl fluoride (Sigma-Aldrich).

    Article Title: Drebrin Regulates Acetylcholine Receptor Clustering and Organization of Microtubules at the Postsynaptic Machinery
    Article Snippet: After checking reflexes and shaving the hindlimb area to be injected, 25 μg DNA (1 μg/μL in PBS) of pEGFP-C1-DbnE-WT or pEGFP-C1-DbnE-142A plasmid was injected into the tibialis anterior muscle using a Hamilton syringe. .. Empty pEGFP-C1 vector (Clontech) was used as a negative control. .. The muscle was electroporated with 10 pulses of electrical current (180 V/cm) of 20 ms each at 1 s intervals using an ECM-830 electroporator (45-0052INT, BTX Harvard Apparatus, USA).

    Negative Control:

    Article Title: Drebrin Regulates Acetylcholine Receptor Clustering and Organization of Microtubules at the Postsynaptic Machinery.
    Article Snippet: After checking reflexes and shaving the hindlimb area to be injected, 25 μg DNA (1 μg/μL in PBS) of pEGFP-C1-DbnE-WT or pEGFP-C1-DbnE-142A plasmid was injected into the tibialis anterior muscle using a Hamilton syringe. .. Empty pEGFP-C1 vector (Clontech) was used as a negative control. .. The muscle was electroporated with 10 pulses of electrical current (180 V/cm) of 20 ms each at 1 s intervals using an ECM-830 electroporator (45-0052INT, BTX Harvard Apparatus, USA).

    Article Title: Drebrin Regulates Acetylcholine Receptor Clustering and Organization of Microtubules at the Postsynaptic Machinery
    Article Snippet: After checking reflexes and shaving the hindlimb area to be injected, 25 μg DNA (1 μg/μL in PBS) of pEGFP-C1-DbnE-WT or pEGFP-C1-DbnE-142A plasmid was injected into the tibialis anterior muscle using a Hamilton syringe. .. Empty pEGFP-C1 vector (Clontech) was used as a negative control. .. The muscle was electroporated with 10 pulses of electrical current (180 V/cm) of 20 ms each at 1 s intervals using an ECM-830 electroporator (45-0052INT, BTX Harvard Apparatus, USA).

    Control:

    Article Title: Golgi-derived CLASP-dependent Microtubules Control Golgi Organization and Polarized Trafficking in Motile Cells
    Article Snippet: Nontargeting siRNA (Dharmacon) was used for controls. .. Empty pEGFP-C1 vector (Clontech) served as a control for circularity quantifications. mCherry plasmid was provided by Dr. R. Tsien (San Diego, CA). .. YFP-GT (YFPGalactosyltransferase; Clontech), mCherry-GT (modified from Clontech), GFP-GM130 (gift from C. Sütterling, Irving, CA), and Cherry-Rab6 (gift from A. Akhmanova, Rotterdam, The Netherlands) were used for Golgi visualization.

    Article Title: Coronin 1C negatively regulates cell-matrix adhesion and motility of intestinal epithelial cells
    Article Snippet: Full length Coronin 1C construct in pEGFP-C1 vector were generated as described previously [ 15 ] and transfected into cells using Lipofectamine 2000 (Invitrogen) according to manufacturers protocol. .. Empty pEGFP-C1 vector (Clontech) was used as a control. .. Human Coronin 1C siGENOME duplex 4 and PTK2 siGENOME SmartPool (Dharmacon, Lafayette, CO) were used to downregulate Coronin 1C and FAK respectively.



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